The researchers apply several techniques to deal with the issue. ICAM-1. Use an inverted intravital microscope with a 3CCD color camera and high-speed video camera and supported laser confocal microscope. This allows a dynamic study of the hepatic microcirculation of the investigation of the mouse subjected to I / R intestine and observes the intestinal I / R imposes impairment vessel diameter, red blood cell velocity sinusoid perfusion and leukocyte rolling and adhesion is obviously alleviated or completely abolished by treatment with R1.. In this study, an animal by ligation of the by ligation of the superior mesenteric artery for 15 min in mice C57/BL established by 30 min reperfusion.
This result is of importance to prevent arose not only for a better understanding of the mechanism of the effect of PN and PN preparations, but also for R1 to liver injury of good I / R.. Second immunofluorescent staining immunofluorescent staining to study endothelial adhesion molecules E-selectin and ICAM-1. Finally, the blood for the detection of the expression of adhesion molecules in leukocytes, and the activity of liver enzymes, including accumulated LDH, ALT and AST , and the concentration of pro-inflammatory mediators such as TNF? IL-6 and monocyte chemotactic protein-1 .This trial uses data via 15,447 children by the age of 17 years of by the Ohio Family Health Survey, meeting, carried out of October 2003 until July 2004. The lead author the study was Joseph Schuchter, an epidemiologist at Cincinnati Children’s. Fairbrother were senior author of.